The objective of the SUSPUFA project is to develop an innovative process for sustainable production of high value n-3 long chain polyunsaturated fatty acids (n-3 LCPUFA), using agro food industry by-products through microalgae cultivation and to evaluate the produced n-3 LCPUFAs as fish oil replacement for incorporation in food formulations. Scientific evidence points out that an appropriate intake of n-3 LCPUFA, widely known as ‘omega-3’, exhibits protective effect on human health. It is recognized that regular consumption of marine n-3 LCPUFA, eicosapentaenoic acid (20:5 n-3; EPA) and docosahexaenoic acid (22:6 n-3; DHA) reduces risk of chronic and inflammatory diseases. The use of fish and fish oil, as main sources of n-3 LCPUFA is increasingly becoming under pressure due to serious public health and ecological concerns. Fish oil quality is dependent on fish diet, it may contain harmful contamination and from sustainability perspective there is a growing issue related to the resilience of current n-3 LCPUFA sources. The main idea of this project is the production of n-3 LCPUFA trough an algae oil production chain, which implies a sustainable approach related to the use of food waste and exploitation of marine n-3 LCPUFA sources. The development of an innovative, cost effective and sustainable process for the production of n-3 LCPUFA will increase the resilience of the current supply chain of n-3 LCPUFA and lower the final cost for these, presently expensive, ingredients, consequently allowing the wider use of n-3 LCPUFA in food consumer goods. Health-promoting algae n-3 LCPUFA produced in a sustainable way will be incorporated in formulations in order to create high added values food products intended to improve the health status of the consumer.